Cytiva HiPrep™ HIC FF 16/10 Columns

Produce fast, preparative separation of proteins and other biomolecules. Cytiva Life Sciences™ HiPrep™ HIC FF 16/10 Columns are high capacity with excellent flow properties, and reliable, reproducible separations.

Supplier:  Cytiva 28936547

Catalog No. 45-002-077

Description

The 20mL HIC columns provide fast, preparative separation of proteins and other biomolecules. They are ready-to-use with four media choices: Phenyl Sepharose 6 Fast Flow (high sub); Phenyl Sepharose 6 Fast Flow (low sub); Butyl Sepharose 4 Fast Flow; and Octyl Sepharose 4 Fast Flow.

Features:

• Offer high capacity, excellent flow properties and reliable, reproducible separations
• Compatible with simple pump-based configurations or chromatography systems (e.g., AKTA)

Specifications (H₂O at room temp.)

• Dimensions: 1.6 x 10cm
• Recommended flowrate: 2-10mL/min. (60-300cm/h)
• Maximum flowrate: 10mL/min. (300cm/hr.)
• Max. pressure over packed bed: 1.5bar (22psi, 0.15MPa)
• Column hardware pressure limit: 5bar (73psi, 0.5MPa)
• Storage: 20% ethanol
• Storage temperature: 4° to 30°C

Specifications

• Buffer: 1M NaOH, 30% Isopropanol, 70% Ethanol, 6M Guanidine Hydrochloride, 30% Aceto nitrile, 1mM HCl
• Bed Volume: 20mL
• Diameter (Metric) Inner: 16mm
• Height (English): 3.93 in.
• Flow Rate: >300cm/hr.
• Ligand Type: Butyl
• pH Range: 3 to 13
• Support Type: 4% Cross-linked Agarose
• Column Type: Ion Exchange Column

• Product Type: HiPrep Butyl Sepharose 4 FF (high sub) column
• Diameter (English) Inner: 0.62 in.
• Quantity: 1 x 20mL
• Height (Metric): 100mm
• Material: Polypropylene
• Max. Pressure: 5 bar
• Particle Size: 45 to 165μm
• Content And Storage: 4° to 30°C
• For Use With (Application): Provide fast, preparative separation of Proteins and other biomolecules by hydrophobic interaction chromato graphy (HIC), initial and intermediate purification, removing bulk impurities