GE Healthcare HiPrep™ HIC FF 16/10 Columns Available on GSA/VA Contract for Federal Government customers only.

Produce fast, preparative separation of proteins and other biomolecules. GE Healthcare Life Sciences™ HiPrep™ HIC FF 16/10 Columns are high capacity with excellent flow properties, and reliable, reproducible separations.

Manufacturer: GE Healthcare 28936547

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Catalog No. 45-002-077

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    Buffer 1M NaOH, 30% Isopropanol, 70% Ethanol, 6M Guanidine Hydrochloride, 30% Aceto nitrile, 1mM HCl
    Quantity 1 x 20mL
    For Use With (Application) Provide fast, preparative separation of Proteins and other biomolecules by hydrophobic interaction chromato graphy (HIC), initial and intermediate purification, removing bulk impurities
    Height (English) 3.93 in.
    Height (Metric) 100mm
    Flow Rate >300cm/hr.
    Material Polypropylene
    Ligand Type Butyl
    Max. Pressure 5 bar
    pH Range 3 to 13
    Particle Size 45 to 165μm
    Support Type 4% Cross-linked Agarose
    Storage Requirements 4° to 30°C
    Bed Volume 20mL
    Diameter (English) Inner 0.62 in.
    Diameter (Metric) Inner 16mm
    Product Type HiPrep Butyl Sepharose 4 FF (high sub) column

    The 20mL HIC columns provide fast, preparative separation of proteins and other biomolecules. They are ready-to-use with four media choices: Phenyl Sepharose 6 Fast Flow (high sub); Phenyl Sepharose 6 Fast Flow (low sub); Butyl Sepharose 4 Fast Flow; and Octyl Sepharose 4 Fast Flow.


    • Offer high capacity, excellent flow properties and reliable, reproducible separations
    • Compatible with simple pump-based configurations or chromatography systems (e.g., AKTA)

    Specifications (H2O at room temp.)

    • Dimensions: 1.6 x 10cm
    • Recommended flowrate: 2-10mL/min. (60-300cm/h)
    • Maximum flowrate: 10mL/min. (300cm/hr.)
    • Max. pressure over packed bed: 1.5bar (22psi, 0.15MPa)
    • Column hardware pressure limit: 5bar (73psi, 0.5MPa)
    • Storage: 20% ethanol
    • Storage temperature: 4° to 30°C

  • Description & Specifications

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