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GE Healthcare HiPrep™ IEX FF 16/10 Columns

Provide fast, reliable and reproducible preparative ion exchange separations

Manufacturer:  GE Healthcare 28936542

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Catalog No. 45-002-070

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DEAE and CM Sepharose™ 6 Fast Flow are prepacked in 20mL HiPrep columns. Ideal for rapid enrichments during initial capture of proteins from starting material.


  • Combine good flowrates and high binding capacity
  • Simple-to-operate
  • Compatible with single-pump configurations and other chromatography systems (e.g., AKTA™)

Specifications (H2O at room temp.)

  • Dimensions: 1.6 x 10cm
  • Recommended flowrate: 2-10mL/min. (60-300cm/h)
  • Maximum flowrate: 10mL/min. (300cm/hr.)
  • Max. pressure over packed bed: 1.5bar (22psi, 0.15MPa)
  • Column hardware pressure limit: 5bar (73psi, 0.5MPa)
  • Storage: 20% ethanol (DEAE,CM), 0.2M sodium acetate in 20% ethanol (SP)
  • Storage temperature: 4° to 30°C



1M NaOH, 8M Urea, 8M Guanidine Hydrochloride, 70% Ethanol
0.62 in.
HiPrep CM Sepharose FF column
Preparative separations of Proteins and other biomolecules using ion exchange (IEX) chromato graphy
1.5 bar
45 to 165μm
4° to 30°C
1 x 20mL
3.93 in.
6 to 10
6% Cross-linked Agarose
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