GE Healthcare HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns Available on GSA/VA Contract for Federal Government customers only.

For reproducible protein purification, separating polysaccarides, macromolecules with extended structures and small plasmids

Manufacturer: GE Healthcare 28935604

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Catalog No. 45-002-990

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    Specifications

    Quantity Each
    Storage Requirements 4° to 30°C
    Flow Rate <1.3mL/min.
    For Use With (Equipment) Low to medium pressure AKTA™ systems or pumped chromato graphy systems
    Bed Volume 120mL
    Product Type HiPrep Sephacryl S-400 HR column
    Diameter (Metric) Inner 1.6cm
    For Use With (Application) Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
    Height (English) 23.62 in.
    Height (Metric) 60cm
    Material Polypropylene
    Max. Pressure 22psi
    Particle Size 25 to 75μm
    pH Range 3 to 11
    Support Type Allyl Dextran and N, N'-methylenebisacrylamide
    Sample Type Polysaccharides, macromolecules with extended structures and even small plasmids

    Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.).

    • Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability
    • Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems
    • Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL)
    • Maximum pressure (over packed bed during operation) is 1.5 bar (22psi)

    Media    

    • Average particle size: 50µm
    • Matrix: Allyl dextran and N, N'-methylenebisacrylamide
    • pH Stability working range: 3 to 11
    • pH Stability cleaning range: 2 to 13
    • Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place)
    • Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm
    Column
    • Material (bed support): Polymethyl-Methacrylate

    HiPrep 16/60 Sephacryl S-400 HR

    • Media:
    • Fractionation [Mr] globular proteins: 2 x 104-8 x 106
    • Exclusion limit [Mr] (globular proteins): »9 x 106 (9000kDa)
    • Fractionation range [Mr] DNA exclusion limit:    271bp
    • Fractionation [Mr] Dextrans: 1 x 104-2 x 106
    HiPrep 16/60 Sephacryl S-500 HR
    • Media:
    • Exclusion limit for Dextrans: 100 x 106
    • Fractionation range [Mr] DNA exclusion limit: 1078bp
    • Fractionation [Mr] Dextrans: 4 x 104-2 x 107

  • Description & Specifications

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