GE Healthcare His Mag Sepharose™ excel Chromatography Media Available on GSA/VA Contract for Federal Government customers only.

His Mag Sepharose excel magnetic beads are designed for small-scale purification and screening of histidine-tagged proteins secreted in eukaryotic cell culture.

Manufacturer: GE Healthcare 17371221

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Catalog No. 45-003-016

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    Specifications

    For Use With (Application) Designed to simplify small-scale purification and screening of His-tagged Proteins_x000D_ secreted in eukaryotic cell culture, used for functional and structural studies research in academia, institutes and discovery Pharma
    pH Range 2 to 12
    Particle Size 63μm
    Product Type His Mag Sepharose Excel
    Quantity 5 x 1mL
    Storage Requirements 4° to 30°C
    Support Type Highly cross-linked spherical Agarose including Magnetite

    Samples that usually cause stripping of metal ions can therefore be added to the medium with retained protein-binding capacity and without pretreatment.

    • His Mag Sepharose excel is a magnetic IMAC medium precharged with nickel ions that are very strongly bound to a chelating ligand.
    • The medium enables an efficient and simplified workflow for capture, purification, and reproducible screening of histidine-tagged proteins.
    • Simplify small-scale purification and expression screening of histidine-tagged proteins secreted in eukaryotic cell culture.
    • Purify histidine-tagged proteins directly from samples that contain metal ion-stripping agents and save time and effort.
    • Increase target protein yield and decrease degradation through reduced and simplified sample handling.
    • Screen histidine-tagged proteins in parallel with high reproducibility and reliable results.
    • Capture histidine-tagged protein from different sample volumes; from low to high milliliter scale.
    • Simplify handling using visible and dense Sepharose-based magnetic beads.
    • Proteins are expressed and secreted into cell culture supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells.
    • The high density of the beads allows rapid capture by magnetic devices while the visibility of the beads ensures reliable collection of the bound histidine-tagged proteins during the purification procedure.
    • The base matrix is based on Sepharose medium with magnetite incorporated.
    • His Mag Sepharose is available as 10% medium slurry in three different pack sizes (2 x 1ml, 5 x 1ml, and 10 x 1ml) and can be used together with different test tubes and magnetic racks.

  • Description & Specifications

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