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GE Healthcare His SpinTrap™ Spin Columns and Kit Available on GSA/VA Contract for Federal Government customers only.

Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. GE Healthcare Life Sciences™ His SpinTrap™ Spin Columns and Kit include prepacked, single-use spin columns.

Manufacturer:  GE Healthcare 28401353

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Catalog No. 45-001-427


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Description

Description

  • Single-use spin columns prepacked with Ni Sepharose High Performance Medium that is precharged with nickel ions; negligible ion leakage
  • One run in standard microcentrifuge takes approximatedly 10 min.
  • Yields up to 750µg purified protein per column
  • Allows direct purification of both clarified and unclarifed cell lysates
  • Also useful for screening large numbers of small-scale bacterial lysates
  • Kit option contains buffer, allowing fast and convenient purifications without time-consuming buffer preparation step

Medium Characteristics:

  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
  • Stable (long-term) in the pH range 3-12
  • Average particle size: 34µm

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Specifications

Specifications

100 μL
1 Pack His SpinTrap and 1 pack His Buffer kit
His SpinTrap
4° to 30°C
600 μl
His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC)
3 to 12
50 x 100 μL
6% Highly Cross-linked Agarose
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