Cytiva His SpinTrap™ Spin Columns and Kit 

Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns and Kit include prepacked, single-use spin columns.

Manufacturer:  Cytiva 28401353

Catalog No. 45-001-427

Description

• Single-use spin columns prepacked with Ni Sepharose High Performance Medium that is precharged with nickel ions; negligible ion leakage
• One run in standard microcentrifuge takes approximatedly 10 min.
• Yields up to 750μg purified protein per column
• Allows direct purification of both clarified and unclarifed cell lysates
• Also useful for screening large numbers of small-scale bacterial lysates
• Kit option contains buffer, allowing fast and convenient purifications without time-consuming buffer preparation step

Medium Characteristics:

• Compatible with a wide range of reducing agents, detergents, denaturants and other additives
• Stable (long-term) in the pH range 3-12
• Average particle size: 34μm

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Specifications

• Bed Volume: 100 μL
• Includes: 1 Pack His SpinTrap and 1 pack His Buffer kit
• Quantity: 50 x 100 μL
• Storage Requirements: 4° to 30°C
• pH Range: 3 to 12

• Description: His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC)
• Product Type: His SpinTrap
• Sample Volume: 600 μl
• Support Type: 6% Highly Cross-linked Agarose