GE Healthcare His SpinTrap™ Spin Columns and Kit Available on GSA/VA Contract for Federal Government customers only.

Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. GE Healthcare Life Sciences™ His SpinTrap™ Spin Columns and Kit include prepacked, single-use spin columns.

Manufacturer: GE Healthcare 28401353

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Catalog No. 45-001-427

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    Specifications

    Bed Volume 100μL
    Includes 1 Pack His SpinTrap and 1 pack His Buffer kit
    pH Range 3 to 12
    Product Type His SpinTrap Spin Column
    Quantity 50 x 100μL
    Storage Requirements 4° to 30°C
    Support Type 6% Highly Cross-linked Agarose

    • Single-use spin columns prepacked with Ni Sepharose High Performance Medium that is precharged with nickel ions; negligible ion leakage
    • One run in standard microcentrifuge takes approximatedly 10 min.
    • Yields up to 750µg purified protein per column
    • Allows direct purification of both clarified and unclarifed cell lysates
    • Also useful for screening large numbers of small-scale bacterial lysates
    • Kit option contains buffer, allowing fast and convenient purifications without time-consuming buffer preparation step

    Medium Characteristics:

    • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
    • Stable (long-term) in the pH range 3-12
    • Average particle size: 34µm

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