GE Healthcare HiScreen™ Blue, Ni and DEAE Sepharose™ Prepacked Fast Flow Columns Available on GSA/VA Contract for Federal Government customers only.

Used for method optimization and parameter screening

Manufacturer: GE Healthcare 28978244

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Catalog No. 45-002-547

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    Buffer 0.1M HCl, 0.1M NaOH, 8M Urea
    Bed Volume 4.7mL
    Diameter (English) Inner 0.3 in.
    Diameter (Metric) Inner 7.7mm
    Product Type HiScreen Ni Sepharose 6 FF column
    Quantity 1 x 4.7mL
    For Use With (Application) Purification of His-tagged Proteins with immobilized Metal ion affinity chromato graphy
    Height (English) 3.93 in.
    Height (Metric) 100mm
    Flow Rate <450cm/hr.
    Material Polypropylene
    Max. Pressure 1.5 bar
    pH Range 3 to 12
    Particle Size 45 to 165μm
    Support Type 6% Cross-linked Agarose
    Storage Requirements 4° to 30°C

    • Based on 6% highly cross-linked agarose with an average bead size of 90µm
    • Processed flow properties and high loading capacities
    • Bed height of 10cm is designed to allow method optimization and parameter screening
    • Easily connected in series to achieve 20cm bed height
    • Small bed volume gives fast results and minimal sample/buffer consumption
    • Combines high capacity with high flow velocity and low backpressure to reduce process cycle times and increase productivity
    • Reproducible results, scalable to BioProcess™ columns packed with the same chromatography media using the same linear fluid velocity

    • Bed volume: 4.7mL
    • Bed dimension: 7.7 x 100mm
    • Matrix: 6% cross-linked agarose beads
    • Average particle size: 90µm
    • Flow velocity: <600cm/hr or <450cm/hr
    • Material (Column hardware and column tube): polypropylene
    • Column I.D.: 7.7mm

    Blue Sepharose

    • Prepacked with BioProcess chromatography medium Blue Sepharose 6 Fast Flow
    • Purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins
    Ni Sepharose
    • Prepacked with Nickel Sepharose 6 Fast Flow
    • Purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC)
    DEAE Sepharose
    • Prepacked with DEAE Sepharose Fast Flow
    • Weak anion exchanger (diethylaminoethyl group) BioProcess™ medium

  • Description & Specifications

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