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Cytiva (Formerly GE Healthcare Life Sciences) HiScreen™ Blue, Ni and DEAE Sepharose™ Prepacked Fast Flow Columns

Used for method optimization and parameter screening

Manufacturer:  Cytiva (Formerly GE Healthcare Life Sciences) 28978244

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Catalog No. 45-002-547


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Description

Description

  • Based on 6% highly cross-linked agarose with an average bead size of 90µm
  • Processed flow properties and high loading capacities
  • Bed height of 10cm is designed to allow method optimization and parameter screening
  • Easily connected in series to achieve 20cm bed height
  • Small bed volume gives fast results and minimal sample/buffer consumption
  • Combines high capacity with high flow velocity and low backpressure to reduce process cycle times and increase productivity
  • Reproducible results, scalable to BioProcess™ columns packed with the same chromatography media using the same linear fluid velocity

  • Bed volume: 4.7mL
  • Bed dimension: 7.7 x 100mm
  • Matrix: 6% cross-linked agarose beads
  • Average particle size: 90µm
  • Flow velocity: <600cm/hr or <450cm/hr
  • Material (Column hardware and column tube): polypropylene
  • Column I.D.: 7.7mm

Blue Sepharose

  • Prepacked with BioProcess chromatography medium Blue Sepharose 6 Fast Flow
  • Purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins
Ni Sepharose
  • Prepacked with Nickel Sepharose 6 Fast Flow
  • Purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC)
DEAE Sepharose
  • Prepacked with DEAE Sepharose Fast Flow
  • Weak anion exchanger (diethylaminoethyl group) BioProcess™ medium

Specifications

Specifications

0.1M HCl, 0.1M NaOH, 8M Urea
0.3 in.
HiScreen Ni Sepharose 6 FF column
Purification of His-tagged Proteins with immobilized Metal ion affinity chromato graphy
100mm
Polypropylene
3 to 12
6% Cross-linked Agarose
4.7mL
7.7mm
1 x 4.7mL
3.93 in.
<450cm/hr.
1.5 bar
45 to 165μm
4° to 30°C
SDS
Documents
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