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GE Healthcare HiScreen™ Capto™ Phenyl (High Sub) Prepacked HIC Column

Hydrophobic interaction chromatography (HIC) media used in the capture and intermediate stages of protein purification, used for method optimization and parameter screening

Manufacturer:  GE Healthcare 28992472

Catalog No. 45-002-549

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  • Highly cross-linked agarose matrix with average bead size of 75µm
  • Has good flow properties and high loading capacities
  • Bed height of 10cm designed to allow method optimization and parameter screening
  • Connect easily in series to achieve 20cm bed height
  • Small bed volume gives fast results and minimal sample/buffer consumption
  • Capto Phenyl (high sub) media is used for initial and intermediate step purifications requiring a chromatography medium with medium to high hydrophobicity
  • Combines high capacity with high flow velocity and low backpressure to reduce process cycle times and increase productivity
  • Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity

  • Bed volume: 4.7mL
  • Bed dimension: 7.7 x 100mm
  • Flow rate: 1.2 to 2.7mL/min.
  • Flow velocity: <600cm/hr.
  • Average particle size: 75µm
  • Degree of substitution: 27µmol/mL gel
  • pH stability cleaning: 2 to 14
  • Pressure/Flow specification: 300kPa at 600cm/hr., 1m diameter column, 20cm bed height
  • Medium: BioProcess
  • pH stability working range: 3 to 13
  • Ligand: phenyl
  • Binding capacity/mL chromatography medium: 27mg BSA /mL (at QB10%)
  • Bed support: filter
  • Material (column hardware and column tube): polypropylene
  • Column I.D.: 7.7mm



Aqueous Buffer
0.3 in.
HiScreen Capto Phenyl (high sub) column
Optimization and parameter screening
4° to 30°C
1 x 4.7mL
3.93 in.
Phenyl (∼27μM/mL drained medium)
3 to 13
Highly Cross-linked Agarose
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