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GE Healthcare HiScreen™ IMAC Sepharose™ 6 Prepacked Fast Flow Columns

Designed for method optimization and parameter screening

Manufacturer:  GE Healthcare 28950517

Catalog No. 45-002-544

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  • Based on 6% highly cross-linked agarose with an average bead size of 90µm
  • Processed flow properties with high binding and loading capacities
  • Bed height of 10cm is designed to allow method optimization and parameter screening
  • Optimal bead size for scale up
  • Charge with the metal of choice for purification of histidine-tagged proteins
  • Reproducible results, scalable to BioProcess™ columns packed with the same chromatography media using the same linear fluid velocity

  • Bed volume: 4.7mL
  • Bed dimension: 7.7 x 100mm
  • Matrix: 6% cross-linked agarose beads
  • Average particle size: 90µm
  • Max. flow velocity: <600cm/hr
  • Material (Column hardware and column tube): polypropylene
  • Column I.D.: 7.7mm



0.01M HCl, 1M NaOH, 70% Acetic Acid, 2% SDS, 30% 2-propanol
0.3 in.
HiScreen IMAC IMAC Sepharose 6 FF column
Optimization and parameter screening
3 to 12
6% Cross-linked Agarose
1 x 4.7mL
3.93 in.
1.5 bar
45 to 165μm
4° to 30°C
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