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Cytiva HiScreen™ Q Sepharose™ and SP Sepharose™ Fast Flow IEX Columns

Designed for method optimization and parameter screening

Manufacturer:  Cytiva 28950513

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Catalog No. 45-002-541

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  • Ready-to-use column for convenient process development optimization and parameter screening
  • Highly cross-linked agarose matrix with average bead size of 90μm
  • Bed height of 10cm designed to allow method optimization and parameter screening
  • 20cm bed height is acheived when it is connected in series
  • Small bed volume (4.7mL) gives fast result and minimal sample/buffer consumption
  • Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity

  • Bed dimension: 7.7 × 100mm
  • Matrix: 6% cross-linked agarose beads
  • Average particle size: 90μm
  • Max. flow velocity: 450cm/hr.
  • Material (column hardware and column tube): polypropylene
  • Column I.D.: 7.7mm

HiScreen Q FF

  • Media:
  • pH stability working range: 2 to 12
  • pH stability cleaning: 2 to 14
  • Ion exchanger type: strong anion exchanger
HiScreen SP FF
  • Media:
  • pH stability working range: 3 to 14
  • pH stability cleaning: 4 to 13
  • Ion exchanger type: strong cation exchanger



0.3 in.
3.93 in.
1.5 bar
HiScreen SP Sepharose FF column
4° to 30°C
4 to 13
1M NaOH, 8M Urea, 8M Guanidine Hydrochloride, 70% Ethanol
Optimization and parameter screening, ion exchange chromato graphy
45 to 165μm
1 x 4.7mL
6% Cross-linked Agarose
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