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Cytiva HiScreen™ Sepharose™ Prepacked High Performance HIC Columns

Designed for method optimization and parameter screening

Manufacturer:  Cytiva 28950516

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Catalog No. 45-002-543

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  • Ready-to-use columns for convenient process development optimization
  • Standard aliphatic/aromatic Hydrophobic Interaction Chromatography media for capture and intermediate purification
  • Bed height of 10cm designed to allow method optimization and parameter screening
  • Easily connected in series to achieve 20cm bed height
  • Small bed volume (4.7mL) gives fast result and minimal sample/buffer consumption
  • Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity

HiScreen Phenyl HP

  • Degree of substitution: ∽25μmol phenyl/mL gel
HiScreen Butyl HP
  • Degree of substitution: ∽50μmol phenyl/mL gel



0.3 in.
3.93 in.
3 bar
HiScreen Phenyl Sepharose HP column
4° to 30°C
3 to 13
1M NaOH, 1M Acetic Acid, 30% Aceto nitrile, 70% Ethanol, 30% Isopropanol, 6M Guanidine Hydrochloride, 8M Ure, 3M Ammoniumsulfate, Ionic Detergents, Non ionic Detergents, Polar Organic Solvents, 2% Sodium Dodectyl Sulfate
Convenient process development optimization parameter screening
24 to 44μm
1 x 4.7mL
6% Cross-linked Agarose
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