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GE Healthcare HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). GE Healthcare Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

Manufacturer:  GE Healthcare 17531902

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Catalog No. 45-002-920

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
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Description

Description

  • Prepacked with precharged Ni Sepharose™ 6 Fast Flow media
  • Column material: polypropylene
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.
  • Protein binding capacity of ~40mg/mL media with negligible nickel ion leakage
  • Compatible with wide range of reducing agents, detergents, denaturants, and other additives
  • Simple operation with syringe, perstaltic pump, or chromatographic system
  • Columns may be connected in series to increase capacity

Medium
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90µm
  • pH stability working range: 3 to 12

Specifications

Specifications

5 mL
16mm
< 20 mL/min
100 x 1 mL
HisTrap FF
100 × 1mL
6% Cross-linked Agarose
0.6 in.
HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Polypropylene
3 to 12
5 bar [0.5 MPa] (70 psi)
4° to 30°C in 20% ethanol
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