GE Healthcare HisTrap™ FF Columns: 16 x 25mm Available on GSA/VA Contract for Federal Government customers only.

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). GE Healthcare Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

Manufacturer: GE Healthcare 17531901

 View more versions of this product

Catalog No. 45-000-336

  Check Availability
Add to Cart


    Bed Volume 1mL
    Support Type 6% Cross-linked Agarose
    Material Polypropylene
    Packaging Quantity 5 × 1mL
    pH Range 3 to 12
    Product Type HisTrap Column
    Pressure 3 bar
    Quantity 5 × 1mL
    Storage Requirements 4° to 30°C in 20% ethanol
    Diameter (English) Inner 0.6 in.
    Diameter (Metric) Inner 16mm
    Flow Rate <20mL/min.

    • Prepacked with precharged Ni Sepharose™ 6 Fast Flow media
    • Column material: polypropylene
    • Bed dimensions: 16 x 25mm
    • Flow velocity: <20mL/min.
    • Protein binding capacity of ~40mg/mL media with negligible nickel ion leakage
    • Compatible with wide range of reducing agents, detergents, denaturants, and other additives
    • Simple operation with syringe, perstaltic pump, or chromatographic system
    • Columns may be connected in series to increase capacity

    • Matrix: 6% cross-linked agarose
    • Average particle size: 90µm
    • pH stability working range: 3 to 12

  • Description & Specifications

Related Products

Product Content Feedback

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit