GE Healthcare HisTrap™ FF Columns: 16 x 25mm Available on GSA/VA Contract for Federal Government customers only.

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). GE Healthcare Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

Manufacturer: GE Healthcare 17-5319-01

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Catalog No. 45-000-336

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    Bed Volume 1mL
    Diameter (English) Inner 0.6 in.
    Diameter (Metric) Inner 16mm
    Flow Rate <20mL/min.
    Material Polypropylene
    Packaging Quantity 5 × 1mL
    pH Range 3 to 12
    Product Type HisTrap Column
    Pressure 3 bar
    Quantity 5 × 1mL
    Storage Requirements 4° to 30°C in 20% ethanol
    Support Type 6% Cross-linked Agarose

    • Prepacked with precharged Ni Sepharose™ 6 Fast Flow media
    • Column material: polypropylene
    • Bed dimensions: 16 x 25mm
    • Flow velocity: <20mL/min.
    • Protein binding capacity of ~40mg/mL media with negligible nickel ion leakage
    • Compatible with wide range of reducing agents, detergents, denaturants, and other additives
    • Simple operation with syringe, perstaltic pump, or chromatographic system
    • Columns may be connected in series to increase capacity

    • Matrix: 6% cross-linked agarose
    • Average particle size: 90µm
    • pH stability working range: 3 to 12

  • Description & Specifications

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