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Cytiva (Formerly GE Healthcare Life Sciences) HisTrap™ FF Columns: 5 x 5mL 
Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.
Manufacturer: Cytiva (Formerly GE Healthcare Life Sciences) 17525501
Description
- Prepacked with precharged Ni Sepharose™ 6 Fast Flow media
- Column material: polypropylene
- Bed dimensions: 7 x 25mm
- Protein binding capacity of ∽40mg/mL media with negligible nickel ion leakage
- Compatible with wide range of reducing agents, detergents, denaturants, and other additives
- Simple operation with syringe, perstaltic pump, or chromatographic system
- Columns may be connected in series to increase capacity
- Matrix: 6% cross-linked agarose
- Average particle size: 90µm
- pH stability working range: 3 to 12
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Specifications
| 1 mL | |
| 7mm | |
| < 4 mL/min | |
| 5 x 5 mL | |
| HisTrap FF | |
| 5 × 5mL | |
| 6% Cross-linked Agarose |
| 0.2 in. | |
| HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). | |
| Polypropylene | |
| 3 to 12 | |
| 5 bar [0.5 MPa] (70 psi) | |
| 4° to 30°C in 20% ethanol |
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