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Cytiva (Formerly GE Healthcare Life Sciences) HisTrap FF Columns: 5 x 5mL
Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose 6 Fast Flow media.
Manufacturer: Cytiva (Formerly GE Healthcare Life Sciences) 17525501
Description
- Prepacked with precharged Ni Sepharose 6 Fast Flow media
- Column material: polypropylene
- Bed dimensions: 7 x 25mm
- Protein binding capacity of ∽40mg/mL media with negligible nickel ion leakage
- Compatible with wide range of reducing agents, detergents, denaturants, and other additives
- Simple operation with syringe, perstaltic pump, or chromatographic system
- Columns may be connected in series to increase capacity
- Matrix: 6% cross-linked agarose
- Average particle size: 90µm
- pH stability working range: 3 to 12
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Specifications
1 mL | |
7mm | |
< 4 mL/min | |
5 x 5 mL | |
HisTrap FF | |
5 × 5mL | |
6% Cross-linked Agarose |
0.2 in. | |
HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). | |
Polypropylene | |
3 to 12 | |
5 bar [0.5 MPa] (70 psi) | |
4° to 30°C in 20% ethanol |
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