Cytiva (Formerly GE Healthcare Life Sciences) HisTrap FF Columns: 5 x 5mL
Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose 6 Fast Flow media.
Manufacturer: Cytiva (Formerly GE Healthcare Life Sciences) 17525501
- Prepacked with precharged Ni Sepharose 6 Fast Flow media
- Column material: polypropylene
- Bed dimensions: 7 x 25mm
- Protein binding capacity of ∽40mg/mL media with negligible nickel ion leakage
- Compatible with wide range of reducing agents, detergents, denaturants, and other additives
- Simple operation with syringe, perstaltic pump, or chromatographic system
- Columns may be connected in series to increase capacity
- Matrix: 6% cross-linked agarose
- Average particle size: 90µm
- pH stability working range: 3 to 12
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|< 4 mL/min|
|5 x 5 mL|
|5 × 5mL|
|6% Cross-linked Agarose|
|HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).|
|3 to 12|
|5 bar [0.5 MPa] (70 psi)|
|4° to 30°C in 20% ethanol|
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