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GE Healthcare HisTrap™ FF Columns: 5 x 5mL Available on GSA/VA Contract for Federal Government customers only.

Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.

Manufacturer:  GE Healthcare 17525501

Catalog No. 45-000-326


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Description

Description

  • Prepacked with precharged Ni Sepharose™ 6 Fast Flow media
  • Column material: polypropylene
  • Bed dimensions: 7 x 25mm
  • Protein binding capacity of ∽40mg/mL media with negligible nickel ion leakage
  • Compatible with wide range of reducing agents, detergents, denaturants, and other additives
  • Simple operation with syringe, perstaltic pump, or chromatographic system
  • Columns may be connected in series to increase capacity
Medium
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90µm
  • pH stability working range: 3 to 12

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Specifications

Specifications

1 mL
7mm
< 4 mL/min
5 x 5 mL
HisTrap FF
5 × 5mL
6% Cross-linked Agarose
0.2 in.
HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Polypropylene
3 to 12
5 bar [0.5 MPa] (70 psi)
4° to 30°C in 20% ethanol
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