Cytiva (Formerly GE Healthcare Life Sciences) HisTrap™ FF Crude Prepacked Columns 

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Catalog Number	Mfr. No.	Quantity	Flow Rate
45-000-335	Cytiva (Formerly GE Healthcare Life Sciences) 17528601	5 x 5mL	< 20 mL/min
45-000-000	Cytiva (Formerly GE Healthcare Life Sciences) 11000458	60mL	< 4 mL/min
45-000-507	Cytiva (Formerly GE Healthcare Life Sciences) 29048631	1mL	< 4 mL/min
45-002-909	Cytiva (Formerly GE Healthcare Life Sciences) 11000459	100 x 1mL	< 4 mL/min

Description

• Prepacked with Ni Sepharose™ 6 Fast Flow media
• Binding capacity approximately 40mg/mL medium with negligible Ni²⁺ leakage
• Maximum pressure: 3 bar
• Column material: polypropylene
• Compatible with wide variety of reducing agents, detergents, denaturants, and other additives
• 1 and 5mL columns

Media

• Matrix: 6% cross-linked agarose
• Average particle size: 90µm
• pH stability working range: 3 to 12

1mL columns

• Bed dimensions: 7 x 25mm
• Flow velocity: <4mL/min.

5mL columns

• Bed dimensions: 16 x 25mm
• Flow velocity: <20mL/min.

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Specifications

• Product Type: HisTrap FF Crude
• Particle Size: 45 to 165μm
• Pressure: 5 bar [0.5 MPa] (70 psi)
• Support Type: 6% Cross-linked Agarose

• Material: Polypropylene
• pH Range: 3 to 12
• Storage Requirements: 4° to 30°C