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Cytiva (Formerly GE Healthcare Life Sciences) HisTrap™ FF Crude Prepacked Columns Available on GSA/VA Contract for Federal Government customers only.

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

$54.75 - $750.00

Specifications

Product Type HisTrap FF Crude
Material Polypropylene
Particle Size 45 to 165μm
pH Range 3 to 12
Pressure 5 bar [0.5 MPa] (70 psi)
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Products 4
Catalog Number Mfr. No. Quantity Flow Rate Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Flow Rate Price Quantity & Availability  
45-000-335 Available on GSA/VA Contract for Federal Government customers only.
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Cytiva (Formerly GE Healthcare Life Sciences)
17528601
5 x 5mL < 20 mL/min
Pack of 5 for $750.00
Add to cart
 
45-000-000 Available on GSA/VA Contract for Federal Government customers only.
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Cytiva (Formerly GE Healthcare Life Sciences)
11000458
60mL < 4 mL/min
Pack of 5 for $209.00
Add to cart
 
45-000-507 Available on GSA/VA Contract for Federal Government customers only.
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Cytiva (Formerly GE Healthcare Life Sciences)
29048631
1mL < 4 mL/min
Each for $54.75
Add to cart
 
45-002-909
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Cytiva (Formerly GE Healthcare Life Sciences)
11000459
100 x 1mL < 4 mL/min This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
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Description

Description

  • Prepacked with Ni Sepharose™ 6 Fast Flow media
  • Binding capacity approximately 40mg/mL medium with negligible Ni2+ leakage
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with wide variety of reducing agents, detergents, denaturants, and other additives
  • 1 and 5mL columns

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90µm
  • pH stability working range: 3 to 12

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.

5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.

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Specifications

Specifications

HisTrap FF Crude
45 to 165μm
5 bar [0.5 MPa] (70 psi)
6% Cross-linked Agarose
Polypropylene
3 to 12
4° to 30°C
SDS
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