GE Healthcare HisTrap HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. GE Healthcare HisTrap HP Prepacked Columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Manufacturer: GE Healthcare 17524801
- Prepacked with Ni Sepharose High Performance medium
- Negligible nickel ion leakage
- Pressure Max: 3 bar
- Column material: polypropylene
- 1 and 5mL columns
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
- Manual operation with syringe, pump or chromatography system
- Matrix: 6% cross-linked agarose
- Average particle size: 34µm
- pH stability working range: 3 to 12
- Bed Dimensions: 7 x 25mm
- Flow velocity: 4mL/min.
- Bed Dimensions: 16 x 25mm
- Flow velocity: 5mL/min.
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|4 mL/min (1 mL), 5 mL/min (5ml)|
|1 x 5 mL|
|HisTrap HP, 1 x 5 mL|
|1 x 5mL|
|6% Highly Cross-linked Agarose|
|HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.|
|3 to 12|
|5 bar [0.5 MPa] (70 psi)|
|4° to 30°C|
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