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GE Healthcare HiTrap™ Chelating HP Columns

Optimize histidine-tagged protein purifications when nickel ions are not the best choice. GE Healthcare Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.

Manufacturer:  GE Healthcare 17040901

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Catalog No. 45-000-061

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  • Uses different kinds of metal ions
  • Binding capacity ~12mg histidine-tagged protein/mL medium
  • Metal ion capacity ~23µmol Cu2+/mL medium
  • Prepacked with Chelating Sepharose High Performance media
  • Iminoacetic acid coupled to a 6% agarose matrix by ether linkage



6M Guanidine Hydrochloride, 8M Urea
0.62 in.
HiTrap chelating HP column
IMAC and subsequent purification of Polyhistidine tagged Proteins
3 bar
4° to 30°C
1 x 5mL
0.98 in.
Iminodiacetic Acid
3 to 13
6% Highly Cross-linked Agarose
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