GE Healthcare HiTrap Chelating HP Columns
Optimize histidine-tagged protein purifications when nickel ions are not the best choice. GE Healthcare Life Sciences HiTrap Chelating HP Columns is prepacked with Chelating Sepharose High Performance.
Manufacturer: GE Healthcare 17040903
- Uses different kinds of metal ions
- Binding capacity ~12mg histidine-tagged protein/mL medium
- Metal ion capacity ~23µmol Cu2+/mL medium
- Prepacked with Chelating Sepharose High Performance media
- Iminoacetic acid coupled to a 6% agarose matrix by ether linkage
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|6M Guanidine Hydrochloride, 8M Urea|
|HiTrap chelating HP column|
|IMAC and subsequent purification of Polyhistidine tagged Proteins|
|4° to 30°C|
|5 x 5mL|
|3 to 13|
|6% Highly Cross-linked Agarose|
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