GE Healthcare HiTrap™ Chelating HP Columns Available on GSA/VA Contract for Federal Government customers only.

Optimize histidine-tagged protein purifications when nickel ions are not the best choice. GE Healthcare Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.

Manufacturer: GE Healthcare 17040903

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Catalog No. 45-000-062

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    Buffer 6M Guanidine Hydrochloride, 8M Urea
    Bed Volume 5mL
    Diameter (English) Inner 0.62 in.
    Diameter (Metric) Inner 16mm
    Product Type HiTrap chelating HP column
    Quantity 5 x 5mL
    For Use With (Application) IMAC and subsequent purification of Polyhistidine tagged Proteins
    Height (English) 0.98 in.
    Height (Metric) 25mm
    Flow Rate <20mL/min.
    Material Polypropylene
    Ligand Type Iminodiacetic Acid
    Max. Pressure 3 bar
    pH Range 3 to 13
    Particle Size 34μm
    Support Type 6% Highly Cross-linked Agarose
    Storage Requirements 4° to 30°C

    • Uses different kinds of metal ions
    • Binding capacity ~12mg histidine-tagged protein/mL medium
    • Metal ion capacity ~23µmol Cu2+/mL medium
    • Prepacked with Chelating Sepharose High Performance media
    • Iminoacetic acid coupled to a 6% agarose matrix by ether linkage

  • Description & Specifications

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