GE Healthcare HiTrap™ MabSelect™ Prepacked Columns

Use these columns in small-scale MAb purification in a continuous bioprocess. GE Healthcare Life Sciences™ HiTrap™ MabSelect™ Prepacked Columns for fast purification of monoclonal antibodies (Mabs) from large sample volumes

Manufacturer: GE Healthcare 28408260

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Catalog No. 45-500-123

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    Specifications

    Quantity Each
    Diameter (Metric) Inner Column 1.6cm
    Diameter (English) Inner Column 0.62 in.
    Binding Capacity ≈40mg Human lgG/mL at 2.4 min. residence time
    Flow Rate <4mL/min.
    For Use With (Application) For fast purification of monoclonal antibodies from large sample volumes, Protein Purification
    Storage Buffer 20% Ethanol
    Storage Requirements 4° to 30°C
    Sample Volume 5mL
    Volume (Metric) Bed 5mL

    Prepacked HiTrap columns for process development, screening of purification conditions, and small-scale purification of Mabs. MabSelect media are suited for the high flowrates and pressures required when scaling up.

    • Oriented coupling of recombinant Protein A ligand to the agarose matrix via an engineered C-terminal cysteine increases IgG binding capacity over other protein A media
    • High capacity, low ligand leakage, and high-flow base matrix allow purification of Mabs at process scale
    • Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification
    • Designed for use with a syringe, a peristaltic pump, or a chromatography system

    MabSelect

    • Allows at least five times higher flowrates than conventional cross-linked agarose of similar porosity
    • Recombinant Protein A ligand promotes specificity of binding to Fc region of IgG; permits one-step purification
    MabSelect Xtra™
    • Same recombinant protein A ligand as MabSelect, but with a smaller particle size and greater porosity, for increased dynamic binding capacity
    • Allows binding from samples with high expression levels of the monoclonal antibody
    • Lowers overall production cost due to the possibility of processing concentrated feedstocks in fewer batches
    MabSelect SuRe™ (Superior Resistance)
    • Akali-tolerant protein A-derived ligand allows the use of up to 0.5M NaOH for cleaning-in-place (CIP)
    • Permits significant cost savings and extension of cycles in regular production

  • Description & Specifications

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