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Cytiva HiTrap™ NHS-Activated HP Columns
Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.
Supplier: Cytiva 17071601
Includes: Complete instructions for use and method optimization, plus a variety of connectors for syringe, peristaltic pump, and chromatography systems
Description
Prepacked with NHS-activated Sepharose™ High Performance Media for coupling ligand via primary amines at close to neutral pH
- Designed for use with a syringe, peristaltic pump, or chromatography system
- Degree of substitution: 10 μmol NHS/mL medium
- Delivered in 100% isopropanol to protect the active groups
- Stable in the pH range 3 to 12, depending upon ligand
- Average particle size: 34μm
Specifications
| 5 x 1mL | |
| 34 μm | |
| 1mL | |
| 0.7cm | |
| 0.98 in. | |
| 10μM NHS groups/mL medium | |
| 42psi | |
| 6% Highly Cross-linked Agarose |
| 4 mL/min. | |
| Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system | |
| NHS-Activated HP Column | |
| Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines | |
| 2.5cm | |
| Polypropylene | |
| 3 to 12 | |
| 4° to 8°C |
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