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Cytiva HiTrap™ Protein A HP Prepacked Columns
Use for purification and isolation of monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants. Cytiva Life Sciences™ HiTrap™ Protein A HP Prepacked Columns are specially designed, together with the medium to provide fast, simple and easy separations in a convenient format.
Supplier: Cytiva 17040203
Description
- Prepacked with Protein A Sepharose High Performance media for purification and fractionation of IgG subclasses
- For use with syringe, pump or chromatography system such as KTA design or FPLC System
- Binds 20mg human IgG/mL medium
- Stable over pH 3 to 9 for use and 2 to 10 for cleaning
- Designed for use with a syringe, a peristaltic pump, or a chromatography system
- Maximum pressure: 3 bar
- Column material: polypropylene
- 1 and 5mL columns
Media
- Ligand: Native protein A
- Matrix: 6% cross-linked agarose
- Average particle size: 34μm
- pH stability working range: 3 to 9
1mL columns
- Bed dimensions: 7 x 25mm
- Flow velocity: <4mL/min.
5mL columns
- Bed dimensions: 16 x 25mm
- Flow velocity: <20mL/min.
Specifications
| 20 mg/mL IgG Medium | |
| Chromatography System, Manual | |
| 4°C to 8°C, 20% Ethanol |
| <4 mL/min. | |
| For syringe, peristaltic pump or a chromatography system | |
| 2 x 1 mL |
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