GE Healthcare HiTrap rProtein A FF Columns
Use for preparative purification of monoclonal and polyclonal antibodies from cell culture supernatants, serum and ascites. GE Healthcare Life Sciences HiTrap rProtein A FF Columns are prepacked with rProtein A Sepharose Fast Flow.
Manufacturer: GE Healthcare 17508001
- Packed with rProtein A Sepharose Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources
- Exhibits similar Fc region specificity to that of native protein A but with enhanced binding capacity and fewer regulatory concerns due to total absence of mammalian culture in ligand production and purification
- Average particle size: 90µm
- Designed for use with a syringe, a peristaltic pump, or a chromatography system
- Available as 1mL and 5mL columns
- Ligand: Recombinant protein A (E.coli)
- Matrix: Highly cross-linked 4% agarose
- Binding capacity: ~50mg human IgG/mL
- pH stability working range: 3 to 10
- Bed dimensions: 7 x 25mm
- Flow velocity: <4mL/min.
- Bed dimensions: 16 x 25mm
- Flow velocity: <20mL/min.
|≈50mg Human IgG/mL|
|4 to 30°C, 20% Ethanol|
|< 20 mL/min|
|For syringe, peristaltic pump or a chromatography system|
|1 x 5 mL|
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