GE Healthcare HiTrap™ rProtein A FF Columns Available on GSA/VA Contract for Federal Government customers only.

Use for preparative purification of monoclonal and polyclonal antibodies from cell culture supernatants, serum and ascites. GE Healthcare Life Sciences™ HiTrap™ rProtein A FF Columns are prepacked with rProtein A Sepharose™ Fast Flow.

Manufacturer: GE Healthcare 17507902

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Catalog No. 45-000-263

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    Specifications

    Quantity 2 x 1mL
    Binding Capacity ≈50mg Human IgG/mL
    Flow Rate <4mL/min.
    For Use With (Application) For preparative purification of monoclonal and polyclonal antibodies from cell culture supernatants, Serum and ascites
    For Use With (Equipment) For syringe, peristaltic pump or a chromatography system
    Storage Requirements 4° to 30°C

    • Packed with rProtein A Sepharose™ Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources
    • Exhibits similar Fc region specificity to that of native protein A but with enhanced binding capacity and fewer regulatory concerns due to total absence of mammalian culture in ligand production and purification
    • Average particle size: 90µm
    • Designed for use with a syringe, a peristaltic pump, or a chromatography system
    • Available as 1mL and 5mL columns
    Media
    • Ligand: Recombinant protein A (E.coli)
    • Matrix: Highly cross-linked 4% agarose
    • Binding capacity: ~50mg human IgG/mL
    • pH stability working range: 3 to 10

    1mL columns

    • Bed dimensions: 7 x 25mm
    • Flow velocity: <4mL/min.
    5mL columns
    • Bed dimensions: 16 x 25mm
    • Flow velocity: <20mL/min.

  • Description & Specifications

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