Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

GE Healthcare HiTrap™ TALON™ Crude Prepacked Columns Available on GSA/VA Contract for Federal Government customers only.

For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).   GE Healthcare Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.

Manufacturer:  GE Healthcare 28953767

 View more versions of this product

Catalog No. 45-002-386


  Check Availability
Add to cart

Specifications

Description

Cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Allows protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

  • Prepacked with cobalt-based chromatography media
  • Binding capacity approximately 7mg MBP
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
  • 1 and 5mL columns

Media
  • Ligand: TALON™ Superflow Cobalt-IMAC
  • Matrix: 6% cross-linked agarose
  • Average particle size: 60 to 160µm
  • pH stability working range: 3 to 12

1mL columns

  • Column I.D.: 7mm
  • Flow velocity: 1 to 4mL/min.

5mL columns
  • Column I.D.: 16
  • Flow velocity: 5 to 20mL/min.

Description & Specifications

Specifications

Up to 20mg Histidine-tagged Protein1
1.6cm
For purifiCatIon of Histidine-tagged recombinant Proteins by immobilized Metal affinity chromatography, used Buffers and easily regenerated with 0.5 M NaOH
Polypropylene
3 bar
5 x 5mL
4 to 8°C
HiTrap Talon™ crude prepacked Column
0.62 in.
1 to 4mL/min.
Talon™ Superflow Cobalt-IMAC
6% Cross-Linked Agarose
5 x 5mL
20% Ethanol
60 to 160μm
3 to 12
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit