GE Healthcare HiTrap™ TALON™ Crude Prepacked Columns Available on GSA/VA Contract for Federal Government customers only.

For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).   GE Healthcare Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.

Manufacturer: GE Healthcare 28953767

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Catalog No. 45-002-386

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    Quantity 5 x 5mL
    Storage Requirements 4 to 8°C
    Binding Capacity Up to 20mg Histidine-tagged Protein1
    Diameter (English) Inner 0.62 in.
    Product Type HiTrap Talon™ crude prepacked Column
    Diameter (Metric) Inner 1.6cm
    For Use With (Application) For purifiCatIon of Histidine-tagged recombinant Proteins by immobilized Metal affinity chromatography, used Buffers and easily regenerated with 0.5 M NaOH
    Flow Rate 1 to 4mL/min.
    Particle Size 60 to 160μm
    Material Polypropylene
    Max. Pressure 3 bar
    Matrix 6% Cross-Linked Agarose
    pH Range 3 to 12
    Storage Buffer 20% Ethanol
    Ligand Type Talon™ Superflow Cobalt-IMAC
    Packaging Quantity 5 x 5mL

    Cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Allows protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

    • Prepacked with cobalt-based chromatography media
    • Binding capacity approximately 7mg MBP
    • Maximum pressure: 3 bar
    • Column material: polypropylene
    • Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
    • 1 and 5mL columns

    • Ligand: TALON™ Superflow Cobalt-IMAC
    • Matrix: 6% cross-linked agarose
    • Average particle size: 60 to 160µm
    • pH stability working range: 3 to 12

    1mL columns

    • Column I.D.: 7mm
    • Flow velocity: 1 to 4mL/min.

    5mL columns
    • Column I.D.: 16
    • Flow velocity: 5 to 20mL/min.

  • Description & Specifications

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