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GE Healthcare HiTrap™ TALON™ Crude Prepacked Columns Available on GSA/VA Contract for Federal Government customers only.

For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).   GE Healthcare Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.

Manufacturer:  GE Healthcare 28953766

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Catalog No. 45-002-385


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Description

Description

Cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Allows protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

  • Prepacked with cobalt-based chromatography media
  • Binding capacity approximately 7mg MBP
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
  • 1 and 5mL columns

Media
  • Ligand: TALON™ Superflow Cobalt-IMAC
  • Matrix: 6% cross-linked agarose
  • Average particle size: 60 to 160µm
  • pH stability working range: 3 to 12

1mL columns

  • Column I.D.: 7mm
  • Flow velocity: 1 to 4mL/min.

5mL columns
  • Column I.D.: 16
  • Flow velocity: 5 to 20mL/min.

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Specifications

Specifications

Up to 20 mg Histidine-tagged Protein1
0.7 cm
For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH
Polypropylene
3 bar
5 x 1 mL
4°C to 8°C
HiTrap Talon crude prepacked Column
0.27 in.
1 to 4 mL/min
Talon Superflow Cobalt-IMAC
6% Cross-Linked Agarose
5 x 1 mL
20% Ethanol
60 - 160 µm
3 to 12
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