Cytiva (Formerly GE Healthcare Life Sciences) HiTrap™ TALON™ Crude Prepacked Columns 

For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).   Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.

Catalog Number	Mfr. No.	Quantity
45-000-502	Cytiva (Formerly GE Healthcare Life Sciences) 29048565	1 x 1 mL
45-002-385	Cytiva (Formerly GE Healthcare Life Sciences) 28953766	5 x 1 mL
45-002-386	Cytiva (Formerly GE Healthcare Life Sciences) 28953767	5 x 5 mL

Description

Cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Allows protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

• Prepacked with cobalt-based chromatography media
• Binding capacity approximately 7mg MBP
• Maximum pressure: 3 bar
• Column material: polypropylene
• Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
• 1 and 5mL columns

Media

• Ligand: TALON™ Superflow Cobalt-IMAC
• Matrix: 6% cross-linked agarose
• Average particle size: 60 to 160µm
• pH stability working range: 3 to 12

1mL columns

• Column I.D.: 7mm
• Flow velocity: 1 to 4mL/min.

5mL columns

• Column I.D.: 16
• Flow velocity: 5 to 20mL/min.

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Specifications

• Particle Size: 60 - 160 µm
• Flow Rate: 1 to 4 mL/min
• Ligand Type: Talon Superflow Cobalt-IMAC
• Matrix: 6% Cross-Linked Agarose
• pH Range: 3 to 12
• Storage Requirements: 4°C to 8°C

• Product Type: HiTrap Talon crude prepacked Column
• For Use With (Application): For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH
• Material: Polypropylene
• Max. Pressure: 3 bar
• Storage Buffer: 20% Ethanol
• Binding Capacity: Up to 20 mg Histidine-tagged Protein1