GE Healthcare illustra™ bacteria genomicPrep Mini Spin Kit Available on GSA/VA Contract for Federal Government customers only.

Designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from Gram-negative (G-) and Gram-positive (G+) bacteria

Manufacturer: GE Healthcare 28904258

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Catalog No. 45-001-493

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    For Use With (Application) Rapid extraction and purification of high-quality genomic DNA from gram-positive and gram-negative bacteria
    Includes 2 x 30mg Proteinase K, 15mL Lysis Buffer type 2, 2 x 1.5mL Lysis Buffer type 3, 2 x 165mL Lysis Buffer type 4, 30mL Wash Buffer type 6, 60mL Elution Buffer type 5, 5 x 50 Illustra™ bacteria mini columns, 5 x 50 collection tubes
    Product Type Kit
    Quantity 50 Preps

    The illustra Bacteria genomicPrep Mini Spin Kit can be used with a wide range of bacterial strains such as DH5a, TOP10, JM109, Bacillus subtilis etc. The kit produces highly pure and intact genomic DNA with OD260/280 values of 1.75-1.85. Use different elution volumes to isolate genomic DNA at concentrations suitable for most downstream applications including restriction enzyme digestion, RT-PCR, and long PCR.

    The method uses a lysis solution in combination with proteinase K to gently release genomic DNA into solution from bacteria cells. The DNA is deproteinated in the extraction solution and the genomic DNA is then bound onto a silica column in the presence of chaotropic solution. Contaminants are removed during wash steps and the purifed DNA is eluted with prewarmed TE solution.

    • Total DNA extraction time under one hour with reduced lysis time
    • Can be used to purify 4 to 8µg of genomic DNA from 2 x 109 bacteria cells with input amounts ranging from 1 x 109 to 4 x 109 bacteria cells
    • Spin columns prepacked with a silica membrane—provide excellent reproducibility and consistency
    • Validated in several downstream applications including RT PCR, long PCR, and restriction digest
    • Color-coded caps for ease of use

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