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GE Healthcare illustra™ GFX 96 PCR Purification Kit Available on GSA/VA Contract for Federal Government customers only.

Simultaneously purify up to 96 PCR products of between 100 bp and 10 kbp in as little as 15 minutes

Manufacturer:  GE Healthcare 28903445

Catalog No. 45-001-488


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Includes: Binding plates; Wash plates; Collection plates and color-coded bottles of capture buffer; Wash buffer; Choice of two elution buffers (Tris-HCl and sterile water); Instruction booklet

Description

Description

  • High yields of pure DNA recovered in a small volume of water or a low-ionic strength buffer
  • Typical recoveries are > 85% for PCR products of 100 bp to 10kb length; Salt removal > 99%
  • Prepacked glass-fiber matrix 96-well plates are validated for use with the Macherey Nagel NucleoVac 96-vacuum manifold
  • No need for ethanol precipitations and hazardous organic extractions

Fluorescent sequencing, microarrays, labeling, hybridization, ligation, and transformation

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Specifications

Specifications

Kit uses glass fiber matrix technology in a 96-well format for highly efficient purification of PCR products. DNA fragments from PCR are captured by the matrix in presence of a chaotropic salt and contaminants are removed by washing matrix.
Glass-fiber matrix 96-well plates binding plates, wash plates, collection plates and color-coded bottles of capture Buffer, Wash Buffer choice of two Elution Buffers (Tris HCl and sterile Water), instruction booklet
96 Well Plates
PCR mixtures, enzyme reactions and DNA solutions
Fluorescent sequencing, microarrays, labeling, hybridization, ligation and transformation
illustra GFX 96 PCR Purification Kit
100 bp-10 kbp
Up to 95% when purifying a 910 bp fragment at a starting concentration of 8.4 ng/μl
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