GE Healthcare illustra™ GFX™ PCR DNA and Gel Band Purification Kit Available on GSA/VA Contract for Federal Government customers only.

Purify and concentrate PCR products or DNA fragments ranging in size from 50 bp to 10 kb. GE Healthcare Life Sciences™ illustra™ GFX™ PCR DNA and Gel Band Purification Kit can be used to purify DNA from reaction volumes up to 100µL or agarose gel slices of up to 900mg.

Manufacturer: GE Healthcare 28903470

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Catalog No. 45-001-489

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    Specifications

    For Use With (Application) Isolation and concentration of DNA fragments (50bp to 10kb) from PCR mixtures, DNA-containing Agarose Gel bands, Enzyme-based DNA modifications and restriction digestions
    Formulation Tris HCl and sterile Water
    Includes GFX™ columns, collection tubes, color-coded bottles of capture Buffer, Wash Buffer, two Elution Buffers
    Quantity 100 Preps

    Typical recoveries range from 60 to 80% for DNA fragments from agarose gel to as high as 95% for PCR products from solution.

    • Rapid and easy-to-use purification method with less than 10 minutes hands-on time
    • Flexible 10 to 50µL elution volume for different DNA concentration needs
    • Improved column design with increased maximum volume holding of up to 800µL liquid volume (950µL without a cap)
    • DNA of exceptional purity (to 99.5%) is ready for direct use in sequencing, PCR, labeling, restriction enzyme digestion, and cloning
    • Visual color indicator added to the capture buffer to ensure optimal pH for maximum DNA binding and recovery
    • Kit combines a versatile chaotropic buffer with a glass fiber matrix supported in a spin-column for the purification of DNA from both solution and agarose gel

  • Description & Specifications

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