Cytiva plasmidPrep Mini Spin Kit

For the rapid isolation of high and low copy number plasmid DNA

Supplier:  Cytiva 28904270

Catalog No. 45-001-500

Includes: Spin columns prepacked with a novel silica-membrane; Suspension, lysis, and neutralization buffers containing chaotropic salts; Wash and elution buffers; Microcentrifuge collection tubes; Full protocol booklet; Detachable, quick reference protocol card

Description

The illustra plasmidPrep Mini Spin Kit uses a simple plasmid DNA purification protocol involving a modified alkaline lysis procedure and a novel silica-based membrane to achieve highly efficient plasmid DNA purification. Instead of organic solvents, the kit contains chaotropic salts to denature protein components and promote selective binding of DNA to the novel silica membrane.

• Total preparation time of less than 10 minutes—representing a 50% reduction in protocol time
• Produces high-quality plasmid DNA with excellent reproducibility for use in cloning, restriction enzyme digestion, PCR amplification, and DNA sequencing
• Increased ease of use provided minimal changes in pipetting volume from one part of the protocol to the next, less centrifugation steps, color-coded kit components, and lack of organic solvents
• The typical plasmid yields from a fresh 1.5 ml culture of E.coli containing a high copy number plasmid is approximately 9 μg
• Can accommodate high and low copy number plasmid purification

Specifications

• Description: Purify high yields of plasmid DNA in less than 10 min. Color-coded tubes and columns simplify the protocol and purified DNA is suitable for all common downstream applications.
• Includes: Spin columns prepacked with a novel Silica membrane, Suspension, Lysis and Neutralization Buffers containing chaotropic salts, Wash and Elution Buffers, microcentrifuge collection tubes, full protocol booklet, Detachable, quick reference protocol card
• Product Type: illustra plasmidPrep Mini Spin Kit
• Sample Volume: 1.5 mL
• Yield: 6-15 μg

• For Use With (Application): Suitable for many downstream applications including—restriction enzyme analysis, ligation, cloning, DNA sequencing and PCRSequencing, PCR, ligation and cloning, restriction digest, enzymatic modification, template for labeled probe generation, transforma
• No. of Reactions: 250 Preps
• Purity: 1.7
• Test Time: Approx. 9 min