GE Healthcare illustra™ Single Cell GenomiPhi™ DNA Amplification Kit

Amplify whole genome DNA from as little as a single cell.  GE Healthcare Lifescience™ illustra™ Single Cell GenomiPhi™ DNA Amplification Kit combines unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference.

Manufacturer: GE Healthcare 29108107

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Catalog No. 45-500-134

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    Specifications

    Final Product Type illustra™ Single Cell GenomiPhi™ DNA Amplification Kit
    Includes Single Cell GenomiPhi Reaction buffer 1 x 275μL, Single Cell GenomiPhi Enzyme mix 1 x 25μL, Single Cell GenomiPhi Amplification mix 1 x 25μL, Single Cell GenomiPhi DTT Solution 1 x 100μL, Single Cell GenomiPhi Lysis buffer 1 x 100μL and Single Cell GenomiPhi Neutralisation buffer 1 x 100μL
    Product Type illustra™ Single Cell GenomiPhi™ DNA Amplification Kit
    Quantity 25 Reactions
    Storage Requirements Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C

    • For whole genome amplification of genomic DNA from as little as a single cell
    • Novel clean-up process supresses background allowing sensitivity down to 1fg of input DNA
    • Simple Isothernal strand displacement (MDA) technology using Phi29 DNA polymerase
    • Amplifications can be completed in less than 3 hours with less than 15 minutes hands-on time
    • Optimized to promote complete cell lysis with uniform denaturation of DNA prior to amplification
    • Reaction time: 2 to 4 hours
    • Product average size: >10kb
    Applications

    For SNP arrays, Next Gen Sequencing, aCGH

    Recommended Storage

    Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C


  • Description & Specifications

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