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GE Healthcare illustra™ TempliPhi™ 2000 Reaction Kit Available on GSA/VA Contract for Federal Government customers only.

Uses isothermal rolling circle amplification (RCA) to efficiently prepare DNA sequencing templates with a formulation designed for higher throughput applications

Manufacturer:  GE Healthcare 28964286

Catalog No. 45-003-001


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Includes: TempliPhi Premix, Denature buffer, Enzyme mix, and Positive control pUC19 DNA (2ng/µL)

Description

Description

  • Simple protocol reduces time, labor and consumables needed for template preparation, and workflow enables easy automation
  • Generate microgram quantities of template DNA from picogram amounts of starting material
  • Amplify DNA from bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks or purified circular (plasmid or M13) DNA
  • Use amplified DNA directly for cycle sequencing without purification
  • Prepare circular DNA templates for cycle sequencing, cloning, and transformation in 4 to 6 h

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Specifications

Specifications

illustra TempliPhi 2000 Reaction Kit
TempliPhi™ premix, denature Buffer, Enzyme mix and positive control pUC19 DNA
2000 Reactions
16-18 h
High-throughput preparation of circular DNA templates for cycle sequencing, cloning, and transformation.
illustra TempliPhi 2000 Reaction Kit
Store Denature buffer and control DNA at -70°C or -15°C to -30°C. Store TempliPhi premix at -70°C. Do not store TempliPhi premix at -15°C to -30°C. Thaw the premix on ice and maintain at 0–4°C during handling. Do not allow premix to warm above 4°C prior
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