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GE Healthcare illustra™ TempliPhi™ Large Construct Kit

Uses isothermal rolling circle amplification (RCA) to efficiently prepare DNA specifically from large constructs (e.g., fosmids and BACs) sufficient for DNA sequencing

Manufacturer:  GE Healthcare 25640080

Catalog No. 45-002-267

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Includes: Sample buffer, Reaction buffer, and Enzyme mix



  • Start from microliter quantities of purified templates, as well as bacterial culture or glycerol stocks
  • Generate 5µg amounts of template DNA for use directly in cycle sequencing reactions without purification
  • Simplified process, 20 minutes hands-on time
  • Amplification is completed in 18 hours with no need for thermal cycling



illustra TempliPhi Large Construct Kit uses isothermal rolling circle amplification (RCA) to efficiently prepare DNA sequencing templates specifically from large DNA vectors (e.g., fosmids and BACs).
9mL Sample Buffer, 9mL Reaction Buffer, 500μL Enzyme mix
1000 reactions
18 h
Sequencing Template Amplification Kits and Reagents
illustra TempliPhi Large Construct Kit
Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C.
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