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GE Healthcare IMAC Sepharose™ High Performance Media

For optimizing purification of histidine-tagged proteins using Immobilized Metal Affinity Chromatography (IMAC) when Ni2+ is not the best choice of metal ion

Manufacturer:  GE Healthcare 17092007

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Catalog No. 45-000-166


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Description

Description

  • 34µm beads of highly cross-linked 6% agarose, to which a chelating group has been covalently coupled
  • Charge with transition metal of choice for optimized selectivity
  • Small bead size for high performance purification
  • High binding capacity: ≥40mg histidine-tagged protein/mL medium when charged with Ni2+
  • Allows more sample to be applied; a more concentrated target protein is obtained more at a lower cost/mg and in less time
  • Stable in pH range 3-12 (long term), and in all commonly used buffers

Avoid use with chelating agents, e.g., EDTA, EGTA, or citrate.

Specifications

Specifications

<300cm/hr.
3 to 12
IMAC Sepharose HP
4° to 30°C
Purification of Polyhistidine tagged Proteins
34μm
100mL
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