GE Healthcare IMAC Sepharose™ High Performance Media Available on GSA/VA Contract for Federal Government customers only.

For optimizing purification of histidine-tagged proteins using Immobilized Metal Affinity Chromatography (IMAC) when Ni2+ is not the best choice of metal ion

Manufacturer: GE Healthcare 17092007

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Catalog No. 45-000-166

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    Specifications

    Flow Rate <300cm/hr.
    For Use With (Application) Purification of Polyhistidine tagged Proteins
    pH Range 3 to 12
    Particle Size 34μm
    Product Type IMAC Sepharose HP
    Quantity 100mL
    Storage Requirements 4° to 30°C

    • 34µm beads of highly cross-linked 6% agarose, to which a chelating group has been covalently coupled
    • Charge with transition metal of choice for optimized selectivity
    • Small bead size for high performance purification
    • High binding capacity: ≥40mg histidine-tagged protein/mL medium when charged with Ni2+
    • Allows more sample to be applied; a more concentrated target protein is obtained more at a lower cost/mg and in less time
    • Stable in pH range 3-12 (long term), and in all commonly used buffers

    Avoid use with chelating agents, e.g., EDTA, EGTA, or citrate.

  • Description & Specifications

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