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GE Healthcare MMP-9 Human, Biotrak assay

Provides a simple, specific and precise quantitative determination of MMP-9 in tissue culture supernatants and homogenates as well as plasma

Manufacturer:  GE Healthcare RPN2614

Catalog No. 45-500-155

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Includes: Anti-MMP-9 Microplate; Assay buffer; Standard; Peroxidase conjugate; Wash buffer; TMB substrate

Description

Description

  • Specific for free pro-MMP-9 and pro-MMP-9 complexed to TIMP-1
  • No cross-reactivity with pro-MMP-1, -2, -3, TIMP-1 and -2
  • Sufficient material for 96 wells. If one standard curve is constructed, 40 unknowns can be measured in duplicate.
  • Range: 1-32ng/mL (4–128 ng/ml with plasma)
  • Sensitivity: 0.6ng/mL
  • Four-hour protocol

Specifications

Specifications

MMP-9
Human
Suitable for use with serum, plasma (EDTA, heparin, citrate) and cell culture supernatant samples Biotrak ELISAs measure MMP protein levels in human serum, plasma, and cell culture samples
Microplate (12 x 8-well strip coated with Anti-MMP-9), 10mL Phosphate Assay Buffer, 64ng Human pro MMP-9 standard(lyophilized), Anti-MMP-9 HRP Conjugate (lyophilized), 12.5mL Wash Buffer, TMB Substrate
Human MMP-9 activity assay
Serum, Plasma (EDTA, Heparin, Citrate), Cell Culture Supernatant
-15 - -30°C
1 to 32ng/mL
MMP-9, Human, Biotrak Assay.
Pipettes or pipetting equipment with disposable polypropylene tips (100 μl, 500 μl, 1 mL and 5 mL); Disposable polypropylene test tubes; Glass measuring cylinders (100 mL and 500 mL); Distilled or deionized water; Spectrophtometric plate reader capable of
PP (Polypropylene)
Each
0.6 ng/ml
4 hr.
Safety and Handling

Safety and Handling

ShelfLife : 1 Month

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