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Cytiva (Formerly GE Healthcare Life Sciences) NHS-Activated Sepharose™ 4 Fast Flow Affinity Media Available on GSA/VA Contract for Federal Government customers only.

Use for coupling amino-containing smaller proteins and peptides.  Cytiva Life Sciences™ NHS-Activated Sepharose™ 4 Fast Flow Affinity Media yields coupled products that are very stable, especially at high pH.

Manufacturer:  Cytiva (Formerly GE Healthcare Life Sciences) 17090601

Catalog No. 45-002-965

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  • Composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides
  • NHS (N-hydroxysuccinimide) coupling forms chemically stable amide bond with ligands containing primary amino groups
  • Resin provides spacer arm to coupled ligand
  • Fast Flow matrix gives high productivity
  • Coupled product is very stable, especially at high pH
  • Available in range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development

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150 to 250 cm/Hoµr
9.84 in.
N-hydroxysuccinimide (NHS) Activated
100 kPa
Stable in commonly used aqueous Buffers provided that the ligand withstand the pH or the chemical environment
2°C to 8°C
NHS-Activated Sepharose 4 fast flow media
For Coupling Amino-Containing Smaller Proteins and Peptides
25 cm
4% Cross-Linked Agarose
25 mL
100% Isopropanol
45 - 165 µm
3 to 13


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