GE Healthcare NHS-Activated Sepharose™ 4 Fast Flow Affinity Media 

Use for coupling amino-containing smaller proteins and peptides.  GE Healthcare Life Sciences™ NHS-Activated Sepharose™ 4 Fast Flow Affinity Media yields coupled products that are very stable, especially at high pH.

Manufacturer:  GE Healthcare 17090601

Catalog No. 45-002-965

Description

• Composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides
• NHS (N-hydroxysuccinimide) coupling forms chemically stable amide bond with ligands containing primary amino groups
• Resin provides spacer arm to coupled ligand
• Fast Flow matrix gives high productivity
• Coupled product is very stable, especially at high pH
• Available in range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development

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Specifications

• Flow Rate: 150 to 250 cm/Hoµr
• Height (English) Bed: 9.84 in.
• Ligand Type: N-hydroxysuccinimide (NHS) Activated
• Max. Pressure: 100 kPa
• Stability: Stable in commonly used aqueous Buffers provided that the ligand withstand the pH or the chemical environment
• Storage Requirements: 2°C to 8°C
• Product Type: NHS-Activated Sepharose 4 fast flow media

• For Use With (Application): For Coupling Amino-Containing Smaller Proteins and Peptides
• Height (Metric) Bed: 25 cm
• Matrix: 4% Cross-Linked Agarose
• Quantity: 25 mL
• Storage Buffer: 100% Isopropanol
• Particle Size: 45 - 165 µm
• pH Range: 3 to 13