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GE Healthcare NHS HP SpinTrap™ Kit

An NHS activated sepharose media permits coupling of a specific affinity ligand to generate an enrichment media for any protein of interest

Manufacturer:  GE Healthcare 28903128

Catalog No. 45-001-480

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Includes: 5mL NHS-Activated Sepharose™ High Performance Medium plus 24 SpinTrap columns



  • Antibody or capturing molecule covalently immobilized via free NH2 groups
  • Simple coupling, carried out near neutral pH
  • Fast and flexible protocol for the capture step, with elution conditions formatted for both electrophoresis and LC-MS analysis workflows
  • Bulk media and empty columns allow for batch coupling; use with a standard microcentrifuge
  • Easy scale-up to HiTrap™ NHS-activated HP Columns



NHS HP SpinTrap kit
Coupling of an affinity molecule with a primary Amine
NHS Sepharose HP
4° to 30°C
Instructions, 5mL NHS activated Sepharose high performance medium in bulk, 24 x empty SpinTrap column
3 to 12
6% Highly Cross-linked Spherical Agarose
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