GE Healthcare Ni Sepharose™ 6 Fast Flow Available on GSA/VA Contract for Federal Government customers only.

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.   GE Healthcare Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.

Manufacturer: GE Healthcare 17531802

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Catalog No. 45-002-985

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    Specifications

    Buffer 0.1M HCl, 0.1M NaOH, 8M Urea
    Product Type Ni Sepharose 6 FF chromato graphy media
    Quantity 100mL
    Height (English) 9.84 in.
    Height (Metric) 250mm
    Flow Rate 600cm/hr.
    Max. Pressure 1 bar
    pH Range 3 to 12
    Particle Size 90μm
    Support Type 6% Cross-linked Agarose
    Storage Requirements 4° to 30°C

    Ni Sepharose 6 Fast Flow is a member of the GE Healthcare range of IMAC chromatography media for capture and intermediate purification.

    • Designed for scaling up purification of histidine-tagged proteins.
    • High protein binding capacity and minimal leakage of Ni2+ ions.
    • As a BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
    • Available in convenient prepacked HisPrep and HisTrap column formats.
    • Composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2+ ions for purification of histidine tagged proteins.
    • The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2+ leakage while providing high capacity.
    • Available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
    • As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

  • Description & Specifications

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