Cytiva Ni Sepharose™ excel

Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

Supplier:  Cytiva 17371201

Catalog No. 45-002-820

Description

• Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity.
• Increase target protein yield and decrease degradation through reduced and simplified sample handling.
• Choose between several formats for screening and preparative purification of histidine-tagged proteins.
• Ni Sepharose excel is an IMAC medium precharged with nickel ions very strongly bound to a chelating ligand, allowing loading of samples that usually cause stripping of metal ions.
• Ni Sepharose excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.

Specifications

• Buffer: 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
• Quantity: 25mL
• pH Range: 2 to 12
• Support Type: 6% Highly Cross-linked Agarose
• For Use With (Application): Capture and purification of His-tagged Proteins secreted into eukaryotic cell culture supernatants by immobilized Metal ion affinity chromatography (IMAC)

• Product Type: Ni Sepharose excel chromato graphy media
• Flow Rate: 150 to 600cm/hr.
• Particle Size: 90μm
• Content And Storage: 4° to 30°C
• Matrix: Ni-Sepharose