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Cytiva Ni Sepharose™ excel Available on GSA/VA Contract for Federal Government customers only.

Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

Manufacturer:  Cytiva 17371203

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Catalog No. 45-003-012

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Ni Sepharose excel enables direct loading of samples without having to perform extensive and time-consuming pretreatment procedures.

  • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity.
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling.
  • Choose between several formats for screening and preparative purification of histidine-tagged proteins.
  • Ni Sepharose excel is an IMAC medium precharged with nickel ions very strongly bound to a chelating ligand, allowing loading of samples that usually cause stripping of metal ions.
  • Ni Sepharose excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.

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0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
Capture and purification of His-tagged Proteins secreted into eukaryotic cell culture supernatants by immobilized Metal ion affinity chromato graphy (IMAC)
Ni Sepharose excel chromato graphy media
4° to 30°C
2 to 12
150 to 600cm/hr.
6% Highly Cross-linked Agarose
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