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Cytiva Ni Sepharose™ excel
Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
$670.00 - $10190.00
Specifications
| Buffer | 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol |
|---|---|
| Product Type | Ni Sepharose excel chromato graphy media |
| Flow Rate | 150 to 600cm/hr. |
| pH Range | 2 to 12 |
| Particle Size | 90μm |
| Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
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| Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
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45-002-820
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Cytiva
17371201 |
25mL |
Each for $670.00
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45-003-011
|
Cytiva
17371202 |
100mL |
Each for $2,590.00
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45-003-012
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Cytiva
17371203 |
500mL |
Each for $10,190.00
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Description
Ni Sepharose excel enables direct loading of samples without having to perform extensive and time-consuming pretreatment procedures.
- Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity.
- Increase target protein yield and decrease degradation through reduced and simplified sample handling.
- Choose between several formats for screening and preparative purification of histidine-tagged proteins.
- Ni Sepharose excel is an IMAC medium precharged with nickel ions very strongly bound to a chelating ligand, allowing loading of samples that usually cause stripping of metal ions.
- Ni Sepharose excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.
Specifications
| 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol | |
| 150 to 600cm/hr. | |
| 90μm | |
| 4° to 30°C |
| Ni Sepharose excel chromato graphy media | |
| 2 to 12 | |
| 6% Highly Cross-linked Agarose | |
| Ni-Sepharose |
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