GE Healthcare Ni Sepharose™ excel Available on GSA/VA Contract for Federal Government customers only.

Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

Manufacturer: GE Healthcare 17371202

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Catalog No. 45-003-011

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    Buffer 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
    Product Type Ni Sepharose excel chromato graphy media
    Quantity 100mL
    For Use With (Application) Capture and purification of His-tagged Proteins secreted into eukaryotic cell culture supernatants by immobilized Metal ion affinity chromato graphy (IMAC)
    Flow Rate 150 to 600cm/hr.
    pH Range 2 to 12
    Particle Size 90μm
    Support Type 6% Highly Cross-linked Agarose
    Storage Requirements 4° to 30°C

    Ni Sepharose excel enables direct loading of samples without having to perform extensive and time-consuming pretreatment procedures.

    • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity.
    • Increase target protein yield and decrease degradation through reduced and simplified sample handling.
    • Choose between several formats for screening and preparative purification of histidine-tagged proteins.
    • Ni Sepharose excel is an IMAC medium precharged with nickel ions very strongly bound to a chelating ligand, allowing loading of samples that usually cause stripping of metal ions.
    • Ni Sepharose excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.

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