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GE Healthcare Ni Sepharose™ excel Available on GSA/VA Contract for Federal Government customers only.

Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

Manufacturer:  GE Healthcare 17371202

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Catalog No. 45-003-011


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Specifications Description & Specifications

Specifications

0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
100mL
150 to 600cm/hr.
90μm
4° to 30°C
Ni Sepharose excel chromato graphy media
Capture and purification of His-tagged Proteins secreted into eukaryotic cell culture supernatants by immobilized Metal ion affinity chromato graphy (IMAC)
2 to 12
6% Highly Cross-linked Agarose
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