GE Healthcare Ni Sepharose excel
Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Manufacturer: GE Healthcare 17371203
|0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol|
|150 to 600cm/hr.|
|4° to 30°C|
|Ni Sepharose excel chromato graphy media|
|Capture and purification of His-tagged Proteins secreted into eukaryotic cell culture supernatants by immobilized Metal ion affinity chromato graphy (IMAC)|
|2 to 12|
|6% Highly Cross-linked Agarose|
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