GE Healthcare nProtein A Sepharose™ 4 Fast Flow Affinity Media Available on GSA/VA Contract for Federal Government customers only.

Used for purification of monoclonal and polyclonal antibodies.   GE Healthcare Lifescience™ nProtein A Sepharose™ 4 Fast Flow Affinity Media contains native protein A coupled to Sepharose 4 Fast Flow.

Manufacturer: GE Healthcare 17528004

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Catalog No. 45-002-983

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    Stability No significant change in chromatographic performance after 1 week storage using 8M Urea, 6M Gua-HCI, 2% Benzyl Alcohol or 20% Ethanol
    Quantity 25mL
    Storage Requirements 2 to 8°C
    Binding Capacity >35mg Human IgG/mL
    Product Type nProtein A Sepharose 4 fast flow media
    For Use With (Application) For the purifiCatIon of monoclonal and polyclonal antibodies at both laboratory and process scale
    Flow Rate 150 to 250cm/Hour
    Particle Size 90μm
    Height (English) Bed 9.84 in.
    Height (Metric) Bed 25cm
    Max. Pressure 100kPa
    Matrix 4% Cross-Linked Agarose
    pH Range 2 to 10
    Storage Buffer 20% Ethanol
    Ligand Type Native Protein A (S. aureus)

    • Low leakage of protein A with nearly twice total IgG binding capacity of Protein A Sepharose CL-4B
    • Used in large-scale FDA-approved processes
    • Manufactured without using animal-derived components
    • Rigidity and high degree of substitution of Sepharose 4 Fast Flow matrix facilitates speedy process of large volumes dilute cell culture fluid
    • High chemical, thermal and mechanical stability
    • Withstands high concentrations of hydrogen bond disrupting agents

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