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GE Healthcare nProtein A Sepharose™ 4 Fast Flow Affinity Media

Used for purification of monoclonal and polyclonal antibodies.   GE Healthcare Lifescience™ nProtein A Sepharose™ 4 Fast Flow Affinity Media contains native protein A coupled to Sepharose 4 Fast Flow.

Manufacturer:  GE Healthcare 17528004

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Catalog No. 45-002-983

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  • Low leakage of protein A with nearly twice total IgG binding capacity of Protein A Sepharose CL-4B
  • Used in large-scale FDA-approved processes
  • Manufactured without using animal-derived components
  • Rigidity and high degree of substitution of Sepharose 4 Fast Flow matrix facilitates speedy process of large volumes dilute cell culture fluid
  • High chemical, thermal and mechanical stability
  • Withstands high concentrations of hydrogen bond disrupting agents


>35 mg Human IgG/mL
For The PurifiCatIon of Monoclonal and Polyclonal Antibodies at Both Laboratory and Process Scale
25 cm
4% Cross-Linked Agarose
25 mL
20% Ethanol
90 µm
2 to 10
150 to 250 cm/Hoµr
9.84 in.
Native Protein A (S. aureus)
100 kPa
No significant change in chromatographic performance after 1 week storage using 8 M Urea, 6 M Gua-HCI, 2% Benzyl Alcohol or 20% Ethanol
2°C to 8°C
nProtein A Sepharose 4 fast flow media
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